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  Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression

Irlbacher, H., Franke, J., Manke, T., Vingron, M., & Ehrenhofer-Murray, A. E. (2005). Control of replication initiation and heterochromatin formation in Saccharomyces cerevisiae by a regulator of meiotic gene expression. Genes & Development, 19(15), 1811-1822. doi:10.1101/gad.334805.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-85D8-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-85D9-1
Genre: Journal Article
Alternative Title : Genes Dev.

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1811.pdf (Any fulltext), 612KB
 
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 Creators:
Irlbacher, Horst1, Author
Franke, Jacqueline1, Author
Manke, Thomas2, Author              
Vingron, Martin3, Author              
Ehrenhofer-Murray, Ann E.4, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
3Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              
4Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554              

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Free keywords: HML-D Hst1 chromatin replication origin ARS
 Abstract: Heterochromatinization at the silent mating-type loci HMR and HML in Saccharomyces cerevisiae is achieved by targeting the Sir complex to these regions via a set of anchor proteins that bind to the silencers. Here, we have identified a novel heterochromatin-targeting factor for HML, the protein Sum1, a repressor of meiotic genes during vegetative growth. Sum1 bound both in vitro and in vivo to HML via a functional element within the HML-E silencer, and sum1{Delta} caused HML derepression. Significantly, Sum1 was also required for origin activity of HML-E, demonstrating a role of Sum1 in replication initiation. In a genome-wide search for Sum1-regulated origins, we identified a set of autonomous replicative sequences (ARS elements) that bound both the origin recognition complex and Sum1. Full initiation activity of these origins required Sum1, and their origin activity was decreased upon removal of the Sum1-binding site. Thus, Sum1 constitutes a novel global regulator of replication initiation in yeast.

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Language(s): eng - English
 Dates: 2005-08-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 272165
DOI: 10.1101/gad.334805
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Title: Genes & Development
  Alternative Title : Genes Dev.
Source Genre: Journal
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Pages: - Volume / Issue: 19 (15) Sequence Number: - Start / End Page: 1811 - 1822 Identifier: ISSN: 0890-9369