Gene expression analysis of pancreatic cell lines reveals genes overexpressed 
in pancreatic cancer

Aldinger, Ingo; Dittert, Dag; Peiper, Matthias; Fusco, Alberto; Chiappetta, Gennaro; Staub, Eike; Loehr, Matthias; Jesnowsk, Ralf; Baretton, Gustavo; Ockert, Detlef; Saeger, Hans-Detlev; Gruetzmann, Robert; Pilarsky, Christian

doi:10.1159/000086537

Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer

Aldinger, I., Dittert, D., Peiper, M., Fusco, A., Chiappetta, G., Staub, E., Loehr, M., Jesnowsk, R., Baretton, G., Ockert, D., Saeger, H.-D., Gruetzmann, R., & Pilarsky, C. (2005). Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer. Pancreatology: Official Journal of the International Association of Pancreatology (IAP), European Pancreatic Club, 5(4-5), 370-379. doi:10.1159/000086537.

Item is 公開

表示: 全項目非表示: 全項目

基本情報

表示: 非表示:

アイテムのパーマリンク: https://hdl.handle.net/11858/00-001M-0000-0010-8614-2 版のパーマリンク: https://hdl.handle.net/11858/00-001M-0000-0010-8615-F

資料種別: 学術論文

その他のタイトル : EPC

ファイル

表示: ファイル

作成者

表示:

非表示:

作成者:
Aldinger, Ingo, 著者
Dittert, Dag, 著者
Peiper, Matthias, 著者
Fusco, Alberto, 著者
Chiappetta, Gennaro, 著者
Staub, Eike¹, 著者
Loehr, Matthias, 著者
Jesnowsk, Ralf, 著者
Baretton, Gustavo, 著者
Ockert, Detlef, 著者
Saeger, Hans-Detlev, 著者
Gruetzmann, Robert, 著者
Pilarsky, Christian, 著者

所属:
1Max Planck Society, ou_persistent13

内容説明

表示:

非表示:

キーワード: Pancreas Cancer Cell line Primary isolates Microarray Thymosin Immunohistochemistry

要旨: Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin beta-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatic tissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.

資料詳細

表示:

非表示:

言語: eng - English

日付: 出版: 2005-06-23

出版の状態: 出版

ページ: -

出版情報: -

目次: -

査読: -

識別子（DOI, ISBNなど）: eDoc: 264875
DOI: 10.1159/000086537

学位: -

訴訟

表示:

Project information

表示:

出版物 1

表示:

非表示:

出版物名: Pancreatology : Official Journal of the International Association of Pancreatology (IAP), European Pancreatic Club

出版物の別名 : EPC

種別: 学術雑誌

著者・編者:

所属:

出版社, 出版地: -

ページ: - 巻号: 5 (4-5) 通巻号: - 開始・終了ページ: 370 - 379 識別子（ISBN, ISSN, DOIなど）: ISSN: 1424-3903

アイテム詳細

基本情報

ファイル

関連URL

作成者

内容説明

資料詳細

関連イベント

訴訟

Project information

出版物 1