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  Long-term maintenance of hematopoietic stem cells does not require contact with embryo-derived stromal cells in cocultures

Oostendorp, R. A., Robin, C., Steinhoff, C., Marz, S., Bräuer, R., Nuber, U. A., et al. (2005). Long-term maintenance of hematopoietic stem cells does not require contact with embryo-derived stromal cells in cocultures. Stem Cells, 23(6), 842-851. doi:10.1634/stemcells.2004-0120.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8621-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8622-2
Genre: Journal Article

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 Creators:
Oostendorp, Robert A.J., Author
Robin, Catherine, Author
Steinhoff, Christine1, Author              
Marz, Stefanie, Author
Bräuer, Rosalinde, Author
Nuber, Ulrike A.2, Author              
Dzierzak, Elaine A., Author
Peschel, Christian, Author
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Hematopoiesis Hematopoietic stem cells AGM Transwell Microenvironment
 Abstract: We recently established that two midgestation-derived stromal clones—UG26-1B6, urogenital ridge–derived, and EL08-1D2, embryonic liver–derived—support the maintenance of murine adult bone marrow and human cord blood hematopoietic repopulating stem cells (HSCs). In this study, we investigate whether direct HSC-stroma contact is required for this stem cell maintenance. Adult bone marrow ckit+ Ly-6C– side population (K6-SP) cells and stromal cells were cocultured under contact or noncontact conditions. These experiments showed that HSCs were maintained for at least 4 weeks in culture and that direct contact between HSCs and stromal cells was not required. To find out which factors might be involved in HSC maintenance, we compared the gene expression profile of EL08-1D2 and UG26-1B6 with four HSC-nonsupportive clones. We found that EL08-1D2 and UG26-1B6 both expressed 21 genes at a higher level, including the putative secreted factors fibroblast growth factor-7, insulin-like growth factor-binding proteins 3 and 4, pleiotrophin, pentaxin-related, and thrombospondin 2, whereas 11 genes, including GPX-3 and HSP27, were expressed at a lower level. In summary, we show for the first time long-term maintenance of adult bone marrow HSCs in stroma noncontact cultures and identify some secreted molecules that may be involved in this support.

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Language(s): eng - English
 Dates: 2005-06-06
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: eDoc: 267906
DOI: 10.1634/stemcells.2004-0120
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Title: Stem Cells
Source Genre: Journal
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Pages: - Volume / Issue: 23 (6) Sequence Number: - Start / End Page: 842 - 851 Identifier: ISSN: 1066-5099