English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-throughput proteomics using matrix-assisted laser desorption/ ionization mass spectrometry

Cramer, R., Gobom, J., & Nordhoff, E. (2005). High-throughput proteomics using matrix-assisted laser desorption/ ionization mass spectrometry. Expert Review of Proteomics, 2(3), 407-420. doi:10.1586/14789450.2.3.407.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-862E-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-862F-8
Genre: Journal Article

Files

show Files
hide Files
:
Cramer et al. - Expert Rev Proteomics.pdf (Any fulltext), 571KB
Name:
Cramer et al. - Expert Rev Proteomics.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: PUBLIC
License:
-

Locators

show

Creators

show
hide
 Creators:
Cramer, Rainer, Author
Gobom, Johan1, Author              
Nordhoff, Eckhard1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: -
 Abstract: It has become evident that the mystery of life will not be deciphered just by decoding its blueprint, the genetic code. In the life and biomedical sciences, research efforts are now shifting from pure gene analysis to the analysis of all biomolecules involved in the machinery of life. One area of these postgenomic research fields is proteomics. Although proteomics, which basically encompasses the analysis of proteins, is not a new concept, it is far from being a research field that can rely on routine and large-scale analyses. At the time the term proteomics was coined, a gold-rush mentality was created, promising vast and quick riches (i.e., solutions to the immensely complex questions of life and disease). Predictably, the reality has been quite different. The complexity of proteomes and the wide variations in the abundances and chemical properties of their constituents has rendered the use of systematic analytical approaches only partially successful, and biologically meaningful results have been slow to arrive. However, to learn more about how cells and, hence, life works, it is essential to understand the proteins and their complex interactions in their native environment. This is why proteomics will be an important part of the biomedical sciences for the foreseeable future. Therefore, any advances in providing the tools that make protein analysis a more routine and large-scale business, ideally using automated and rapid analytical procedures, are highly sought after. This review will provide some basics, thoughts and ideas on the exploitation of matrix-assisted laser desorption/ ionization in biological mass spectrometry – one of the most commonly used analytical tools in proteomics – for high-throughput analyses.

Details

show
hide
Language(s): eng - English
 Dates: 2005-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 268566
DOI: 10.1586/14789450.2.3.407
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Expert Review of Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 2 (3) Sequence Number: - Start / End Page: 407 - 420 Identifier: ISSN: 1478-9450