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  How to improve quality assurance in fluorometry : fluorescence-inherent sources of error and suited fluorescence standards

Resch-Genger, U., Hoffmann, K., Nietfeld, W., Engel, A., Neukammer, J., Nitschke, R., et al. (2005). How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence standards. Journal of Fluorescence, 15(3), 337-362. doi:10.1007/s10895-005-2630-3.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8667-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8668-8
Genre: Journal Article
Alternative Title : J Fluorescence

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Resch-Genger et al. - J Fluoresc.pdf (Any fulltext), 605KB
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Resch-Genger et al. - J Fluoresc.pdf
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 Creators:
Resch-Genger, U., Author
Hoffmann, K.1, Author              
Nietfeld, W.2, Author              
Engel, A., Author
Neukammer, J., Author
Nitschke, R., Author
Ebert, P., Author
Macdonald, R., Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Fluorescence; standard; calibration; microarray; in vivo imaging; flow cytometry
 Abstract: The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for frequently used photoluminescence techniques ranging from conventional macro- and microfluorometry over fluorescence microscopy and flow cytometry to microarray technology as well as in vivo fluorescence imaging. Particularly, the need for and requirements on fluorescence standards for the characterization and performance validation of fluorescence instruments, to enhance the comparability of fluorescence data, and to enable quantitative fluorescence analysis are discussed. Special emphasis is dedicated to spectral fluorescence standards and fluorescence intensity standards.

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Language(s): eng - English
 Dates: 2005-05
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 273092
DOI: 10.1007/s10895-005-2630-3
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Title: Journal of Fluorescence
  Alternative Title : J Fluorescence
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 15 (3) Sequence Number: - Start / End Page: 337 - 362 Identifier: ISSN: 1053-0509