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  Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry

Giavalisco, P., Nordhoff, E., Kreitler, T., Klöppel, K.-D., Lehrach, H., Klose, J., et al. (2005). Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. PROTEOMICS, 5(7), 1902-1913. doi:10.1002/pmic.200401062.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8692-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8693-6
Genre: Journal Article

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Giavalisco et al. - Proteomics.pdf (Any fulltext), 713KB
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 Creators:
Giavalisco, Patrick1, Author
Nordhoff, Eckhard2, Author              
Kreitler, Thomas1, Author
Klöppel, Klaus-Dieter3, Author              
Lehrach, Hans2, Author              
Klose, Joachim1, Author
Gobom, Johan2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: Arabidopsis thaliana; Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry; Two-dimensional gel electrophoresis
 Abstract: In the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis.

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Language(s): eng - English
 Dates: 2005-04-06
 Publication Status: Published in print
 Pages: -
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 Identifiers: eDoc: 271267
DOI: 10.1002/pmic.200401062
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Title: PROTEOMICS
Source Genre: Journal
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Pages: - Volume / Issue: 5 (7) Sequence Number: - Start / End Page: 1902 - 1913 Identifier: ISSN: 1615-9853