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  Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

Gutjahr, C., Murphy, D., Lueking, A., Koenig, A., Janitz, M., O'Brien, J., et al. (2005). Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling. Genomics, 85(3), 285-296. doi:10.1016/j.ygeno.2004.11.005.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8723-9 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8724-7
Genre: Journal Article

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 Creators:
Gutjahr, Claudia1, Author
Murphy, Derek, Author
Lueking, Angelika1, Author
Koenig, Andrea2, Author           
Janitz, Michal3, Author           
O'Brien, John, Author
Korn, Bernhard, Author
Horn, Sabine1, Author
Lehrach, Hans3, Author           
Cahill, Dolores J., Author
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: TH1; cDNA expression library; Automation; Mouse; High-density protein arrays; Microarrays; Protein chips; Serum profiling; SLE; Autoantibody
 Abstract: The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse TH1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.

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Language(s): eng - English
 Dates: 2005-01-07
 Publication Status: Issued
 Pages: -
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 Identifiers: eDoc: 271548
DOI: 10.1016/j.ygeno.2004.11.005
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Title: Genomics
Source Genre: Journal
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Pages: - Volume / Issue: 85 (3) Sequence Number: - Start / End Page: 285 - 296 Identifier: ISSN: 0888-7543