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  The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1

Rabus, R., Kube, M., Heider, J., Beck, A., Heitmann, K., Widdel, F., et al. (2005). The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1. Archives of Microbiology, 183(1), 27-36. doi:10.1007/s00203-004-0742-9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-874B-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-874C-F
Genre: Journal Article
Alternative Title : Arch Microbiol

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2bxyj4ln0cmubf73.pdf (Any fulltext), 366KB
 
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 Creators:
Rabus, Ralf1, Author
Kube, Michael2, Author              
Heider, Johann, Author
Beck, Alfred3, Author              
Heitmann, Katja1, Author
Widdel, Friedrich1, Author
Reinhardt, Richard2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
3Computing (Head: Donald Buczek/Peter Marquardt), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479667              

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Free keywords: Complete genome Anaerobic degradation Aromatic compounds Denitrifying bacterium Betaproteobacteria
 Abstract: Recent research on microbial degradation of aromatic and other refractory compounds in anoxic waters and soils has revealed that nitrate-reducing bacteria belonging to the Betaproteobacteria contribute substantially to this process. Here we present the first complete genome of a metabolically versatile representative, strain EbN1, which metabolizes various aromatic compounds, including hydrocarbons. A circular chromosome (4.3 Mb) and two plasmids (0.21 and 0.22 Mb) encode 4603 predicted proteins. Ten anaerobic and four aerobic aromatic degradation pathways were recognized, with the encoding genes mostly forming clusters. The presence of paralogous gene clusters (e.g., for anaerobic phenylacetate oxidation), high sequence similarities to orthologs from other strains (e.g., for anaerobic phenol metabolism) and frequent mobile genetic elements (e.g., more than 200 genes for transposases) suggest high genome plasticity and extensive lateral gene transfer during metabolic evolution of strain EbN1. Metabolic versatility is also reflected by the presence of multiple respiratory complexes. A large number of regulators, including more than 30 two-component and several FNR-type regulators, indicate a finely tuned regulatory network able to respond to the fluctuating availability of organic substrates and electron acceptors in the environment. The absence of genes required for nitrogen fixation and specific interaction with plants separates strain EbN1 ecophysiologically from the closely related nitrogen-fixing plant symbionts of the Azoarcus cluster. Supplementary material on sequence and annotation are provided at the Web page http://www.micro-genomes.mpg.de/ebn1/.

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Language(s): eng - English
 Dates: 2005-01-01
 Publication Status: Published in print
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 Identifiers: eDoc: 272391
DOI: 10.1007/s00203-004-0742-9
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Title: Archives of Microbiology
  Alternative Title : Arch Microbiol
Source Genre: Journal
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Pages: - Volume / Issue: 183 (1) Sequence Number: - Start / End Page: 27 - 36 Identifier: ISSN: 0302-8933