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  An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., et al. (2004). An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature, 432(7020), 1036-1040. doi:10.1038/nature03159.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-876D-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-876E-3
Genre: Journal Article

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 Creators:
Kittler, Ralf, Author
Putz, Gabriele, Author
Pelletier, Laurence, Author
Poser, Ina, Author
Heninger, Anne-Kristin, Author
Drechsel, David, Author
Fischer, Steffi, Author
Konstantinova, Irena, Author
Habermann, Bianca, Author
Grabner, Hannes, Author
Yaspo, Marie-Laure1, Author              
Himmelbauer, Heinz2, Author              
Korn, Bernd, Author
Neugebauer, Karla, Author
Pisabarro, Maria Teresa, Author
Buchholz, Frank, Author
Affiliations:
1Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes are specifically silenced through degradation of messenger RNAs; this process is mediated by homologous double-stranded (ds)RNA molecules. In invertebrates, long dsRNAs have been used for genome-wide screens and have provided insights into gene functions. Because long dsRNA triggers a nonspecific interferon response in many vertebrates, short interfering (si)RNA or short hairpin (sh)RNAs must be used for these organisms to ensure specific gene silencing. Here we report the generation of a genome-scale library of endoribonuclease-prepared short interfering (esi)RNAs from a sequence-verified complementary DNA collection representing 15,497 human genes. We used 5,305 esiRNAs from this library to screen for genes required for cell division in HeLa cells. Using a primary high-throughput cell viability screen followed by a secondary high content videomicroscopy assay, we identified 37 genes required for cell division. These include several splicing factors for which knockdown generates mitotic spindle defects. In addition, a putative nuclear-export terminator was found to speed up cell proliferation and mitotic progression after knockdown. Thus, our study uncovers new aspects of cell division and establishes esiRNA as a versatile approach for genomic RNAi screens in mammalian cells.

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Language(s): eng - English
 Dates: 2004-12-23
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 230601
DOI: 10.1038/nature03159
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 432 (7020) Sequence Number: - Start / End Page: 1036 - 1040 Identifier: ISSN: 0028-0836