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  High-throughput gene silencing using cell arrays

Vanhecke, D., & Janitz, M. (2004). High-throughput gene silencing using cell arrays. Oncogene, 23(51), 8353-8358. doi:10.1038/sj.onc.1208027.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8785-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8786-B
Genre: Journal Article

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 Creators:
Vanhecke, Dominique1, Author
Janitz, Michal2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: RNA interference; microarray; reverse transfection; cell array
 Abstract: A recently established transfected cell array (TCA) technology has opened new experimental dimensions in the field of functional genomics. Cell arrays allow for transfection of several thousands different DNA molecules in microarray format. The effects of overexpression of hundreds of proteins on cellular physiology can be observed in a single experiment. The TCA technique has also found its application in RNA interference (RNAi) research. Small interfering RNAs (siRNA) as well as plasmid expressing short hairpin RNAs can be transferred into the cells through the process of reverse transfection. The silencing of numerous genes in spatially separated manner can be thus monitored. This review will provide an overview on current concepts concerning combination of cell array and RNAi for high-throughput loss-of-function studies.

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Language(s): eng - English
 Dates: 2004-11-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 230840
DOI: 10.1038/sj.onc.1208027
 Degree: -

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Title: Oncogene
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 23 (51) Sequence Number: - Start / End Page: 8353 - 8358 Identifier: ISSN: 0950-9232