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  Systematic delineation of a calmodulin peptide interaction

Hultschig, C., Hecht, H.-J., & Frank, R. (2004). Systematic delineation of a calmodulin peptide interaction. Journal of Molecular Biology, 343(3), 559-568. doi:10.1016/j.jmb.2004.08.012.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8793-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8794-B
Genre: Journal Article
Alternative Title : J Mol Biol

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 Creators:
Hultschig, Claus1, Author
Hecht, Hans-Jürgen, Author
Frank, Ronald, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: SPOT synthesis; protein–peptide interaction; calmodulin; peptide array; structure activity relationship
 Abstract: We present a comprehensive profile of amino acid side-chain constraints in a calmodulin (CaM) peptide complex. These data were obtained from the analysis of calmodulin binding to an array of all single substitution analogues as well as N- and C-terminal truncations of the skMLCK derived M13 peptide ligand. The experimentally derived binding data were evaluated with respect to the known 3D-structure of the CaM/M13 complex. Besides an almost perfect agreement between the measured affinities and the structural data, the unexpected high-affine Asn5Ala variant of the M13* peptide described by Montigiani et al. could be verified. In contrast to other reports our data clearly support the postulate of the minor and major hydrophobic anchors of this calcium dependent interaction.

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Language(s): eng - English
 Dates: 2004-10-22
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 230222
DOI: 10.1016/j.jmb.2004.08.012
 Degree: -

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Title: Journal of Molecular Biology
  Alternative Title : J Mol Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 343 (3) Sequence Number: - Start / End Page: 559 - 568 Identifier: ISSN: 0022-2836