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  The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L

Soukenik, M., Diehl, A., Leidert, M., Sievert, V., Büssow, K., Leitner, D., et al. (2004). The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L. FEBS Letters, 576(3), 358-362. doi:10.1016/j.febslet.2004.09.037.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8795-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8796-7
Genre: Journal Article
Alternative Title : FEBS Lett

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 Creators:
Soukenik, Michael, Author
Diehl, Anne, Author
Leidert, Martina, Author
Sievert, Volker1, Author              
Büssow, Konrad1, Author              
Leitner, Dietmar, Author
Labudde, Dirk, Author
Ball, Linda J., Author
Lechner, Annette, Author
Nägler, Dorit K., Author
Oschkinat, Hartmut, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: SEP; p97; p47; Cathepsin L; NMR; Protein structure
 Abstract: The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171–270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains' function was formulated and pursued in binding experiments with cysteine proteases. The SEP domain was found to be a reversible competitive inhibitor of cathepsin L with a Ki of 1.5 small mu, GreekM. The binding of G1-S2-p47(171–270) to cathepsin L was mapped by biochemical assays and the binding interface was investigated by NMR chemical shift perturbation experiments.

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Language(s): eng - English
 Dates: 2004-10-22
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 230810
DOI: 10.1016/j.febslet.2004.09.037
 Degree: -

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Title: FEBS Letters
  Alternative Title : FEBS Lett
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 576 (3) Sequence Number: - Start / End Page: 358 - 362 Identifier: ISSN: 0014-5793