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  Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA

Borodina, T. A., Lehrach, H., & Soldatov, A. V. (2004). Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA. Analytical Biochemistry, 333, 309-319. doi:10.1016/j.ab.2004.05.032.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-87A2-9 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-87A3-7
Genre: Journal Article
Alternative Title : Anal Biochem

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 Creators:
Borodina, Tatiana A.1, Author           
Lehrach, Hans2, Author           
Soldatov, Aleksey V.1, Author           
Affiliations:
1Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479657              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: SNP; Genotyping; TaqMan; Mutation; Detection
 Abstract: In this article, we describe a genotyping approach applicable to both individual and multiplexed single nucleotide polymorphism (SNP) analysis, based on a ligation detection reaction (LDR) performed directly on genomic DNA. During the ligation, the biallelic state of the SNP locus is converted into a bimarker state of ligated detector oligonucleotides. The state of the markers is then determined by a 5′-nuclease assay (TaqMan) with universal fluorescent probes. The LDR-TaqMan method was successfully applied for the genotyping of 30 SNP loci of Arabidopsis thaliana. The technology is cost-effective, needs no locus-specific optimization, requires minimal manipulations, and has very good potential for automation.

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Language(s): eng - English
 Dates: 2004-10-15
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 229517
DOI: 10.1016/j.ab.2004.05.032
 Degree: -

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Title: Analytical Biochemistry
  Alternative Title : Anal Biochem
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 333 Sequence Number: - Start / End Page: 309 - 319 Identifier: ISSN: 0003-2697