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  Hypervariable and highly divergent intron–exon organizations in the chordate Oikopleura dioica

Edvardsen, R. B., Lerat, E., Maeland, A. D., Flåt, M., Tewari, R., Jensen, M. F., et al. (2004). Hypervariable and highly divergent intron–exon organizations in the chordate Oikopleura dioica. Journal of Molecular Evolution, 59(4), 448-457. doi:10.1007/s00239-004-2636-5.

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Genre: Journal Article
Alternative Title : J Mol Evol

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 Creators:
Edvardsen, Rolf B., Author
Lerat, Emmanuelle, Author
Maeland, Anne Dorthea, Author
Flåt, Mette, Author
Tewari, Rita, Author
Jensen, Marit F., Author
Lehrach, Hans1, Author              
Reinhardt, Richard2, Author              
Seo, Hee-Chan, Author
Chourrou, Daniel, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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Free keywords: Intron; Conversion; agr-Tubulin; Hox; Ribosomal protein; Urochordate; Oikopleura
 Abstract: Oikopleura dioica is a pelagic tunicate with a very small genome and a very short life cycle. In order to investigate the intron–exon organizations in Oikopleura, we have isolated and characterized ribosomal protein EF-1agr, Hox, and agr-tubulin genes. Their intron positions have been compared with those of the same genes from various invertebrates and vertebrates, including four species with entirely sequenced genomes. Oikopleura genes, like Caenorhabditis genes, have introns at a large number of nonconserved positions, which must originate from late insertions or intron sliding of ancient insertions. Both species exhibit hypervariable intron–exon organization within their agr-tubulin gene family. This is due to localization of most nonconserved intron positions in single members of this gene family. The hypervariability and divergence of intron positions in Oikopleura and Caenorhabditis may be related to the predominance of short introns, the processing of which is not very dependent upon the exonic environment compared to large introns. Also, both species have an undermethylated genome, and the control of methylation-induced point mutations imposes a control on exon size, at least in vertebrate genes. That introns placed at such variable positions in Oikopleura or C. elegans may serve a specific purpose is not easy to infer from our current knowledge and hypotheses on intron functions. We propose that new introns are retained in species with very short life cycles, because illegitimate exchanges including gene conversion are repressed. We also speculate that introns placed at gene-specific positions may contribute to suppressing these exchanges and thereby favor their own persistence.

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Language(s): eng - English
 Dates: 2004-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 230182
DOI: 10.1007/s00239-004-2636-5
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Title: Journal of Molecular Evolution
  Alternative Title : J Mol Evol
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 59 (4) Sequence Number: - Start / End Page: 448 - 457 Identifier: ISSN: 0022-2844