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  BRCA1-mediated repression of select X chromosome genes

Jazaeri, A. A., Chandramouli, G. V., Aprelikova, O., Nuber, U. A., Sotiriou, C., Liu, E. T., et al. (2004). BRCA1-mediated repression of select X chromosome genes. Journal of Translational Medicine, 2, 32-32. doi:10.1186/1479-5876-2-32.

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 Creators:
Jazaeri, Amir A, Author
Chandramouli, Gadisetti VR, Author
Aprelikova, Olga, Author
Nuber, Ulrike A1, Author
Sotiriou, Christos, Author
Liu, Edison T, Author
Ropers, Hans-Hilger2, Author           
Yee, Cindy J, Author
Boyd, Jeff, Author
Barrett, J Carl, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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 Abstract: Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Re-expression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus.

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Language(s): eng - English
 Dates: 2004-09-21
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 224724
DOI: 10.1186/1479-5876-2-32
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Title: Journal of Translational Medicine
Source Genre: Journal
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Pages: - Volume / Issue: 2 Sequence Number: - Start / End Page: 32 - 32 Identifier: ISSN: 1479-5876