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  Automated solid-phase extraction for purification of single nucleotide polymorphism genotyping products prior to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometric analysis

Sauer, S., Kepper, P., Smyra, A., Dahl, A., Ferse, F.-T., Lehrach, H., et al. (2004). Automated solid-phase extraction for purification of single nucleotide polymorphism genotyping products prior to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometric analysis. Journal of Chromatography A, 1049(1-2), 9-16. doi:10.1016/j.chroma.2004.07.069.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-87C8-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-87C9-4
Genre: Journal Article
Alternative Title : J Chromatogr A

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 Creators:
Sauer, Sascha1, Author              
Kepper, Pamela2, Author
Smyra, Anett2, Author
Dahl, Andreas3, Author              
Ferse, Falk-Thilo, Author
Lehrach, Hans3, Author              
Reinhardt, Richard4, Author              
Affiliations:
1Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479662              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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Free keywords: Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry; Single nucleotide polymorphism; Solid-phase extraction; Genotyping; Purification
 Abstract: In this article, we describe the application of a novel micro elution solid-phase extraction method for purification of short stretches of DNA products of single nucleotide polymorphisms (SNPs) prior to MALDI mass spectrometry analysis. An important feature of our method is that the purification columns containing a copolymer of divinylbenzene and N-vinylpyrrolidone can be used several times thereby significantly reducing costs. We implemented this DNA purification technology into a fully automated procedure including molecular biology, MALDI sample preparation, automated mass spectrometric analysis and SNP allele calling by software. Due to the facile purification protocol, the methodology shown could furthermore be used for other applications where efficient medium to high-throughput purification of nucleic acids is required.

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Language(s): eng - English
 Dates: 2004-09-17
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 230781
DOI: 10.1016/j.chroma.2004.07.069
 Degree: -

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Title: Journal of Chromatography A
  Alternative Title : J Chromatogr A
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 1049 (1-2) Sequence Number: - Start / End Page: 9 - 16 Identifier: ISSN: 0021-9673