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  A catalog of human cDNA expression clones and its application to structural genomics

Büssow, K., Quedenau, C., Sievert, V., Tischer, J., Scheich, C., Seitz, H., et al. (2004). A catalog of human cDNA expression clones and its application to structural genomics. Genome Biology, 5(9), R71-R71. doi:10.1186/gb-2004-5-9-r71.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-87EB-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-87EC-5
Genre: Journal Article

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 Creators:
Büssow, Konrad1, Author              
Quedenau, Claudia2, Author              
Sievert, Volker1, Author              
Tischer, Janett3, Author
Scheich, Christoph3, Author
Seitz, Harald1, Author              
Hieke, Brigitte3, Author
Niesen, Frank H., Author
Götz, Frank, Author
Harttig, Ulrich, Author
Lehrach, Hans1, Author              
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554              
3Max Planck Society, ou_persistent13              

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 Abstract: We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

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Language(s): eng - English
 Dates: 2004-08-17
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 229527
DOI: 10.1186/gb-2004-5-9-r71
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Title: Genome Biology
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 5 (9) Sequence Number: - Start / End Page: R71 - R71 Identifier: ISSN: 1465-6914