A catalog of human cDNA expression clones and its application to structural 
genomics

Büssow, Konrad; Quedenau, Claudia; Sievert, Volker; Tischer, Janett; Scheich, Christoph; Seitz, Harald; Hieke, Brigitte; Niesen, Frank H.; Götz, Frank; Harttig, Ulrich; Lehrach, Hans

doi:10.1186/gb-2004-5-9-r71

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A catalog of human cDNA expression clones and its application to structural genomics

Büssow, K., Quedenau, C., Sievert, V., Tischer, J., Scheich, C., Seitz, H., et al. (2004). A catalog of human cDNA expression clones and its application to structural genomics. Genome Biology, 5(9), R71-R71. doi:10.1186/gb-2004-5-9-r71.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-87EB-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-87EC-5

Genre: Journal Article

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Büssow, Konrad¹, Author
Quedenau, Claudia², Author
Sievert, Volker¹, Author
Tischer, Janett³, Author
Scheich, Christoph³, Author
Seitz, Harald¹, Author
Hieke, Brigitte³, Author
Niesen, Frank H., Author
Götz, Frank, Author
Harttig, Ulrich, Author
Lehrach, Hans¹, Author

Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550
2Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554
3Max Planck Society, ou_persistent13

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Abstract: We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

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Language(s): eng - English

Dates: Date issued: 2004-08-17

Publication Status: Issued

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Identifiers: eDoc: 229527
DOI: 10.1186/gb-2004-5-9-r71

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Title: Genome Biology

Source Genre: Journal

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Pages: - Volume / Issue: 5 (9) Sequence Number: - Start / End Page: R71 - R71 Identifier: ISSN: 1465-6914