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  The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation

Abdelkhalek, H. B., Beckers, A., Schuster-Gossler, K., Pavlova, M. N., Burkhardt, H., Lickert, H., et al. (2004). The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation. Genes and Development, 18(14), 1725-1736. doi:10.1101/gad.303504.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8808-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-880A-C
Genre: Journal Article
Alternative Title : Genes Dev


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Abdelkhalek, Hanaa Ben, Author
Beckers, Anja, Author
Schuster-Gossler, Karin, Author
Pavlova, Maria N., Author
Burkhardt, Hannelore, Author
Lickert, Heiko, Author
Rossant, Janet, Author
Reinhardt, Richard1, Author              
Schalkwyk, Leonard C.2, Author
Müller, Ines3, Author              
Herrmann, Bernhard G.4, Author              
Ceolin, Marcelo, Author
Rivera-Pomar, Rolando, Author
Gossler, Achim, Author
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
2Max Planck Society, ou_persistent13              
3Molecular Cytogenetics (Reinhard Ullmann), Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479645              
4Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              


Free keywords: Notochord development; Not gene; homeodomain protein
 Abstract: The floating head (flh) gene in zebrafish encodes a homeodomain protein, which is essential for notochord formation along the entire body axis. flh orthologs, termed Not genes, have been isolated from chick and Xenopus, but no mammalian ortholog has yet been identified. Truncate (tc) is an autosomal recessive mutation in mouse that specifically disrupts the development of the caudal notochord. Here, we demonstrate that truncate arose by a mutation in the mouse Not gene. The truncate allele (Nottc) contains a point mutation in the homeobox of Not that changes a conserved Phenylalanine residue in helix 1 to a Cysteine (F20C), and significantly destabilizes the homeodomain. Reversion of F20C in one allele of homozygous tc embryonic stem (ES) cells is sufficient to restore normal notochord formation in completely ES cell-derived embryos. We have generated a targeted mutation of Not by replacing most of the Not coding sequence, including the homeobox with the eGFP gene. The phenotype of NoteGFP/eGFP, NoteGFP/tc, and Nottc/tc embryos is very similar but slightly more severe in NoteGFP/eGFP than in Nottc/tc embryos. This confirms allelism of truncate and Not, and indicates that tc is not a complete null allele. Not expression is abolished in Foxa2 and T mutant embryos, suggesting that Not acts downstream of both genes during notochord development. This is in contrast to zebrafish embryos, in which flh interacts with ntl (zebrafish T) in a regulatory loop and is essential for development of the entire notochord, and suggests that different genetic control circuits act in different vertebrate species during notochord formation.


Language(s): eng - English
 Dates: 2004-07-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 229464
DOI: 10.1101/gad.303504
 Degree: -



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Source 1

Title: Genes and Development
  Alternative Title : Genes Dev
Source Genre: Journal
Publ. Info: -
Pages: - Volume / Issue: 18 (14) Sequence Number: - Start / End Page: 1725 - 1736 Identifier: -