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  A first generation physical map of the medaka genome in BACs essential for positional cloning and clone-by-clone based genomic sequencing

Khorasani, M. Z., Hennig, S., Imre, G., Asakawa, S., Palczewski, S., Berger, A., et al. (2004). A first generation physical map of the medaka genome in BACs essential for positional cloning and clone-by-clone based genomic sequencing. Mechanisms of Development, 121(7-8), 903-913. doi:10.1016/j.mod.2004.03.024.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8822-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8823-1
Genre: Journal Article
Alternative Title : Mech Dev

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 Creators:
Khorasani, Maryam Zadeh1, Author
Hennig, Steffen2, Author              
Imre, Gabriele1, Author
Asakawa, Shuichi, Author
Palczewski, Stefanie2, Author              
Berger, Anja3, Author              
Hori, Hiroshi, Author
Naruse, Kiyoshi, Author
Mitani, Hiroshi, Author
Shima, Akihiro, Author
Lehrach, Hans2, Author              
Wittbrodt, Jochen, Author
Kondoh, Hisato, Author
Shimizu, Nobuyoshi, Author
Himmelbauer, Heinz2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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Free keywords: Medaka; Bacterial artificial chromosome; Positional cloning; Physical map; Genome sequencing
 Abstract: In order to realize the full potential of the medaka as a model system for developmental biology and genetics, characterized genomic resources need to be established, culminating in the sequence of the medaka genome. To facilitate the map-based cloning of genes underlying induced mutations and to provide templates for clone-based genomic sequencing, we have created a first-generation physical map of the medaka genome in bacterial artificial chromosome (BAC) clones. In particular, we exploited the synteny to the closely related genome of the pufferfish, Takifugu rubripes, by marker content mapping. As a first step, we clustered 103,144 public medaka EST sequences to obtain a set of 21,121 non-redundant sequence entities. Avoiding oversampling of gene-dense regions, 11,254 of EST clusters were successfully matched against the draft sequence of the fugu genome, and 2363 genes were selected for the BAC map project. We designed 35mer oligonucleotide probes from the selected genes and hybridized them against 64,500 BAC clones of strains Cab and Hd-rR, representing 14-fold coverage of the medaka genome. Our data set is further supplemented with 437 results generated from PCR-amplified inserts of medaka cDNA clones and BAC end-fragment markers. Our current, edited, first generation medaka BAC map consists of 902 map segments that cover about 74% of the medaka genome. The map contains 2721 markers. Of these, 2534 are from expressed sequences, equivalent to a non-redundant set of 2328 loci. The 934 markers (724 different) are anchored to the medaka genetic map. Thus, genetic map assignments provide immediate access to underlying clones and contigs, simplifying molecular access to candidate gene regions and their characterization.

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Language(s): eng - English
 Dates: 2004-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 230598
DOI: 10.1016/j.mod.2004.03.024
 Degree: -

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Title: Mechanisms of Development
  Alternative Title : Mech Dev
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 121 (7-8) Sequence Number: - Start / End Page: 903 - 913 Identifier: ISSN: 0925-4773