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  Gene expression changes in the course of neural progenitor cell differentiation

Gurok, U., Steinhoff, C., Lipkowitz, B., Ropers, H.-H., Scharff, C., & Nuber, U. A. (2004). Gene expression changes in the course of neural progenitor cell differentiation. Journal of Neuroscience, 24(26), 5982-6002. doi:10.1523/JNEUROSCI.0809-04.2004.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-882E-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-882F-9
Genre: Journal Article
Alternative Title : J. Neurosci.

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 Creators:
Gurok, Ulf1, Author
Steinhoff, Christine2, Author              
Lipkowitz, Bettina3, Author              
Ropers, Hans-Hilger4, Author              
Scharff, Constance4, Author              
Nuber, Ulrike A.4, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
3Familial Cognitive Disorders (Luciana Musante), Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479644              
4Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: development; differentiation; hybridization; ventricle; stem cell; microarray; subventricular zone; rostral migratory stream; neurosphere
 Abstract: The molecular changes underlying neural progenitor differentiation are essentially unknown. We applied cDNA microarrays with 13,627 clones to measure dynamic gene expression changes during the in vitro differentiation of neural progenitor cells that were isolated from the subventricular zone of postnatal day 7 mice and grown in vitro as neurospheres. In two experimental series in which we withdrew epidermal growth factor and added the neurotrophins Neurotrophin-4 or BDNF, four time points were investigated: undifferentiated cells grown as neurospheres, and cells 24, 48, and 96 hr after differentiation. Expression changes of selected genes were confirmed by semiquantitative RT-PCR. Ten different groups of gene expression dynamics obtained by cluster analysis are described. To correlate selected gene expression changes to the localization of respective proteins, we performed immunostainings of cultured neurospheres and of brain sections from adult mice. Our results provide new insights into the genetic program of neural progenitor differentiation and give strong hints to as yet unknown cellular communications within the adult subventricular zone stem cell niche.

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Language(s): eng - English
 Dates: 2004-06-30
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 224393
DOI: 10.1523/JNEUROSCI.0809-04.2004
 Degree: -

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Title: Journal of Neuroscience
  Alternative Title : J. Neurosci.
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 24 (26) Sequence Number: - Start / End Page: 5982 - 6002 Identifier: ISSN: 0270-6474