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  Protein arrays from cDNA expression libraries

Weiner, H., Faupel, T., & Büssow, K. (2004). Protein arrays from cDNA expression libraries. In E. Fung (Ed.), Protein arrays: methods and protocols (pp. 1-14). Totowa, NJ: Humana Press.

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 Creators:
Weiner, Hendrik, Author
Faupel, Thomas1, Author
Büssow, Konrad2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: Protein array; cDNA expression library; high-density spotting; clone array; protein antigen; protein function; protein-protein interaction; posttranslational modification; high-throughput screening
 Abstract: This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the array for binding proteins. After producing the library and decollating the expression clones, one can pick thousands of expression clones with a laboratory robot and can deposit them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting material for the construction of a high-density protein array. This array is constructed by spotting the expression clones onto a protein-binding membrane. Following cell growth and induction of protein expression on the membrane, the cell spots are lysed and their recombinant protein immobilized on the membrane. The so-constructed array carries thousands of proteins without the need to clone, express, and spot individual proteins. Such arrays allow one to screen for numerous protein functions in a high-throughput manner.

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Language(s): eng - English
 Dates: 2004-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 230843
 Degree: -

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Title: Protein arrays : methods and protocols
Source Genre: Book
 Creator(s):
Fung, Eric, Editor
Affiliations:
-
Publ. Info: Totowa, NJ : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 14 Identifier: -

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Title: Methods in molecular biology
Source Genre: Series
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Pages: - Volume / Issue: 264 Sequence Number: - Start / End Page: - Identifier: -