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  Chromosome termini of the monocot plant Othocallis siberica are maintained by telomerase, which specifically synthesizes vertebrate-type telomere sequences

Weiss-Schneeweiss, H., Riha, K., Jang, C. G., Puizina, J., Scherthan, H., & Schweizer, D. (2004). Chromosome termini of the monocot plant Othocallis siberica are maintained by telomerase, which specifically synthesizes vertebrate-type telomere sequences. The Plant Journal, 37(4), 484-493. doi:10.1046/j.1365-313X.2003.01974.x.

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Genre: Journal Article
Alternative Title : Plant J

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 Creators:
Weiss-Schneeweiss, Hanna, Author
Riha, Karel, Author
Jang, Chang Gee, Author
Puizina, Jasna, Author
Scherthan, Harry1, Author           
Schweizer, Dieter, Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Arabidopsis-type telomeric sequences - FISH - Othocallis siberica - telomerase - TRAP assay - vertebrate-type telomeric sequences
 Abstract: Lack of Arabidopsis-type T3AG3 telomere sequences has recently been reported for the majority of investigated taxa of the monocot order Asparagales. In order to investigate this phenomenon in more detail, we conducted extensive cytogenetic and molecular analyses of the telomeres in Othocallis siberica, a member of this order. Terminal restriction fragment analysis together with Bal31 exonuclease assay showed that chromosome termini in O. siberica are formed by long stretches (more than 10 kbp) of vertebrate-type T2AG3 repeats. In addition, telomerase activity specifically synthesising (T2AG3)n sequence was detected in O. siberica protein extracts by telomerase repeat amplification protocol (TRAP). Fluorescence in situ hybridisation (FISH) revealed the presence of the vertebrate-type T2AG3 telomere sequences at all chromosome termini and at a few additional regions of O. siberica chromosomes, whereas Arabidopsis-type T3AG3 DNA and peptide nucleic acid (PNA) probes did not hybridise to chromosomes of Othocallis, except for polymorphic blocks in chromosomes 2 (interstitial) and 4 (terminal). These interstitial/terminal regions are apparently composed of large blocks of (T2AG3)n and (T3AG3)n DNA and represent a unique example of interspersion of two types of telomeric repeats within one genome. This may be a reflection of the recent evolutionary switch from Arabidopsis- to vertebrate-type telomeric repeats in this plant group.

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Language(s): eng - English
 Dates: 2004-02-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 225753
DOI: 10.1046/j.1365-313X.2003.01974.x
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Title: The Plant Journal
  Alternative Title : Plant J
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 37 (4) Sequence Number: - Start / End Page: 484 - 493 Identifier: ISSN: 1365-313X