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  Generation, annotation, evolutionary analysis, and database integration of 20,000 unique sea urchin EST clusters

Poustka, A. J., Groth, D., Hennig, S., Thamm, S., Cameron, A., Beck, A., et al. (2003). Generation, annotation, evolutionary analysis, and database integration of 20,000 unique sea urchin EST clusters. Genome Research, 13(12), 2736-2746.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8957-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8958-3
Genre: Journal Article
Alternative Title : Genome Res.

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 Creators:
Poustka, Albert J.1, Author              
Groth, Detlef2, Author
Hennig, Steffen3, Author              
Thamm, Sabine4, Author              
Cameron, Andrew, Author
Beck, Alfred5, Author              
Reinhardt, Richard6, Author              
Herwig, Ralf7, Author              
Panopoulou, Georgia1, Author              
Lehrach, Hans3, Author              
Affiliations:
1Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
4Human Chromosome 21 (Marie-Laure Yaspo), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479652              
5Computing (Head: Donald Buczek/Peter Marquardt), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479667              
6High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              
7Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              

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 Abstract: Together with the hemichordates, sea urchins represent basal groups of nonchordate invertebrate deuterostomes that occupy a key position in bilaterian evolution. Because sea urchin embryos are also amenable to functional studies, the sea urchin system has emerged as one of the leading models for the analysis of the function of genomic regulatory networks that control development. We have analyzed a total of 107,283 cDNA clones of libraries that span the development of the sea urchin Strongylocentrotus purpuratus. Normalization by oligonucleotide fingerprinting, EST sequencing and sequence clustering resulted in an EST catalog comprised of 20,000 unique genes or gene fragments. Around 7000 of the unique EST consensus sequences were associated with molecular and developmental functions. Phylogenetic comparison of the identified genes to the genome of the urochordate Ciona intestinalis indicate that at least one quarter of the genes thought to be chordate specific were already present at the base of deuterostome evolution. Comparison of the number of gene copies in sea urchins to those in chordates and vertebrates indicates that the sea urchin genome has not undergone extensive gene or complete genome duplications. The established unique gene set represents an essential tool for the annotation and assembly of the forthcoming sea urchin genome sequence. All cDNA clones and filters of all analyzed libraries are available from the resource center of the German genome project at http://www.rzpd.de

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Language(s): eng - English
 Dates: 2003-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 173948
ISI: 000186920200024
 Degree: -

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Title: Genome Research
  Alternative Title : Genome Res.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 13 (12) Sequence Number: - Start / End Page: 2736 - 2746 Identifier: ISSN: 1088-9051