English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island

Schubbe, S., Kube, M., Scheffel, A., Wawer, C., Heyen, U., Meyerdierks, A., et al. (2003). Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island. Journal of Bacteriology, 185(19), 5779-5790. doi:10.1128/JB.185.19.5779-5790.2003.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : J. Bacteriol.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Schubbe, Sabrina1, Author              
Kube, Michael1, Author              
Scheffel, André, Author
Wawer, Cathrin, Author
Heyen, Udo, Author
Meyerdierks, Anke, Author
Madkour, Mohamed H., Author
Mayer, Frank, Author
Reinhardt, Richard1, Author              
Schuler, Dirk1, Author              
Affiliations:
1High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

Content

show
hide
Free keywords: -
 Abstract: Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria.

Details

show
hide
Language(s): eng - English
 Dates: 2003-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 173936
ISI: 000185493500014
DOI: 10.1128/JB.185.19.5779-5790.2003
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Bacteriology
  Alternative Title : J. Bacteriol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 185 (19) Sequence Number: - Start / End Page: 5779 - 5790 Identifier: ISSN: 0021-9193