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  A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome

Hansen, J., Floss, T., Van Sloun, P., Fuchtbauer, E.-M., Vauti, F., Arnold, H.-H., Schnutgen, F., Wurst, W., von Melchner, H., & Ruiz, P. (2003). A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome. Proceedings of the National Academy of Sciences of the United States of America, 100(17), 9918-9922.

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資料種別: 学術論文
その他のタイトル : Proc. Natl. Acad. Sci. U. S. A.

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 作成者:
Hansen, Jens, 著者
Floss, Thomas, 著者
Van Sloun, Petra, 著者
Fuchtbauer, Ernst-Martin, 著者
Vauti, Franz, 著者
Arnold, Hans-Hennig, 著者
Schnutgen, Frank, 著者
Wurst, Wolfgang, 著者
von Melchner, Harald, 著者
Ruiz, Patricia1, 著者
所属:
1Max Planck Society, ou_persistent13              

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 要旨: A major challenge of the postgenomic era is the functional characterization of every single gene within the mammalian genome. In an effort to address this challenge, we assembled a collection of mutations in mouse embryonic stem (ES) cells, which is the largest publicly accessible collection of such mutations to date. Using four different gene-trap vectors, we generated 5,142 sequences adjacent to the gene-trap integration sites (gene-trap sequence tags; http://genetrap.de) from >11,000 ES cell clones. Although most of the gene-trap vector insertions occurred randomly throughout the genome, we found both vector-independent and vector-specific integration "hot spots." Because >50% of the hot spots were vector-specific, we conclude that the most effective way to saturate the mouse genome with gene-trap insertions is by using a combination of gene-trap vectors. When a random sample of gene-trap integrations was passaged to the germ line, 59% (17 of 29) produced an observable phenotype in transgenic mice, a frequency similar to that achieved by conventional gene targeting. Thus, gene trapping allows a large-scale and cost-effective production of ES cell clones with mutations distributed throughout the genome, a resource likely to accelerate genome annotation and the in vivo modeling of human disease.

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言語: eng - English
 日付: 2003-08-19
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): eDoc: 127827
ISI: 000184926000054
 学位: -

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出版物 1

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出版物名: Proceedings of the National Academy of Sciences of the United States of America
  出版物の別名 : Proc. Natl. Acad. Sci. U. S. A.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: -
ページ: - 巻号: 100 (17) 通巻号: - 開始・終了ページ: 9918 - 9922 識別子(ISBN, ISSN, DOIなど): ISSN: 0027-8424