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  Ligation-based synthesis of oligonucleotides with block structure

Borodina, T. A., Lehrach, H., & Soldatov, A. V. (2003). Ligation-based synthesis of oligonucleotides with block structure. Analytical Biochemistry, 318(2), 309-313.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-89EF-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-89F0-B
Genre: Journal Article
Alternative Title : Anal. Biochem.

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 Creators:
Borodina, Tatiana A.1, Author              
Lehrach, Hans2, Author              
Soldatov, Aleksey V.1, Author              
Affiliations:
1Technology Development(Alexey Soldatov), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479657              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: oligonucleotides, primers, padlock probe, block structure
 Abstract: We describe here a method for the synthesis of oligonucleotides with block structure (padlock probes, primers for multiplex polymerase chain reaction (PCR), and ligation-independent cloning), based on the ligation of presynthesized parts by T4 DNA ligase. The advantages of this approach are: (i) suitability of the technology for any producer—from synthesis company to laboratory, (ii) high quality and adjustable scale of synthesis, and (iii) possibility of including any modified bases inexpensively in the common part of the oligonucleotide. Clear difference of sizes of products and substrates makes the synthesis amenable to automation. For large series of padlock probes, the price per one primer approaches the price of the locus-specific parts. We demonstrate the application of this method to two different tasks: preparative-scale production of padlock probes and small-scale synthesis of PCR primers.

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Language(s): eng - English
 Dates: 2003-07-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 173627
ISI: 000183785000018
 Degree: -

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Title: Analytical Biochemistry
  Alternative Title : Anal. Biochem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 318 (2) Sequence Number: - Start / End Page: 309 - 313 Identifier: ISSN: 0003-2697