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  Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella

Schuhmann, B., Seitz, V., Vilcinskas, A., & Podsiadlowski, L. (2003). Cloning and expression of gallerimycin, an antifungal peptide expressed in immune response of greater wax moth larvae, Galleria mellonella. Archives of Insect Biochemistry and Physiology, 53(3), 125-133. doi:10.1002/arch.10091.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8A12-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8A13-6
Genre: Journal Article
Alternative Title : Arch. Insect Biochem. Physiol.

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 Creators:
Schuhmann, Berit, Author
Seitz, Volkhard1, Author              
Vilcinskas, Andreas, Author
Podsiadlowski, Lars, Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

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Free keywords: insect immunity, antifungal peptide, gallerimycin
 Abstract: A novel defensin-like peptide was identified in the greater wax moth, Galleria mellonella. It was discovered in a haemocyte cDNA bank enriched with transcripts upregulated after immune challenge via subtractive hybridisation and suppressive PCR. The deduced amino acid sequence of the defensin-like peptide exhibits similarities to the antifungal peptides drosomycin from Drosophila melanogaster and heliomicin from Heliothis virescens. Therefore, it has been termed gallerimycin. Upregulation of gallerimycin after stimulation of the immune system by LPS-injection was demonstrated by quantitative real-time PCR. A full-size cDNA was cloned and overexpressed in Escherichia coli Origami cells in order to obtain a functional peptide with disulfide bridges. The recombinant peptide was active against the entomopathogenic fungus Metarhizium anisopliae, but not against yeast, gram-negative and gram-positive bacteria.

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Language(s): eng - English
 Dates: 2003-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 175361
ISI: 000183814400004
DOI: 10.1002/arch.10091
 Degree: -

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Title: Archives of Insect Biochemistry and Physiology
  Alternative Title : Arch. Insect Biochem. Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 53 (3) Sequence Number: - Start / End Page: 125 - 133 Identifier: ISSN: 0739-4462