English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  New evidence for genome-wide duplications at the origin of vertebrates using an amphioxus gene set and completed animal genomes

Panopoulou, G., Hennig, S., Groth, D., Krause, A., Poustka, A. J., Herwig, R., et al. (2003). New evidence for genome-wide duplications at the origin of vertebrates using an amphioxus gene set and completed animal genomes. Genome Research, 13(6), 1056-1066.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8A3C-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8A3D-A
Genre: Journal Article
Alternative Title : Genome Res.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Panopoulou, Georgia1, Author              
Hennig, Steffen2, Author              
Groth, Detlef3, Author
Krause, Antje3, Author
Poustka, Albert J.1, Author              
Herwig, Ralf4, Author              
Vingron, Martin5, Author              
Lehrach, Hans2, Author              
Affiliations:
1Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3Max Planck Society, ou_persistent13              
4Bioinformatics (Ralf Herwig), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479648              
5Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              

Content

show
hide
Free keywords: -
 Abstract: The 2R hypothesis predicting two genome duplications at the origin of vertebrates is highly controversial. Studies published so far include limited sequence data from organisms close to the hypothesized genome duplications. Through the comparison of a gene catalog from amphioxus, the closest living invertebrate relative of vertebrates, to 3453 single-copy genes orthologous between Caenorhabditis elegans (C), Drosophila melanogaster (D), and Saccharomyces cerevisiae (Y), and to Ciona intestinalis ESTs, mouse, and human genes, we show with a large number of genes that the gene duplication activity is significantly higher after the separation of amphioxus and the vertebrate lineages, which we estimate at 650 million years (Myr). The majority of human orthologs of 195 CDY groups that could be dated by the molecular clock appear to be duplicated between 300 and 680 Myr with a mean at 488 million years ago (Mya). We detected 485 duplicated chromosomal segments in the human genome containing CDY orthologs, 331 of which are found duplicated in the mouse genome and within regions syntenic between human and mouse, indicating that these were generated earlier than the human–mouse split. Model based calculations of the codon substitution rate of the human genes included in these segments agree with the molecular clock duplication time-scale prediction. Our results favor at least one large duplication event at the origin of vertebrates, followed by smaller scale duplication closer to the bird–mammalian split. [Supplementary material is available online at www.genome.org. The cDNA clones used in the EST sequencing are available from http://www.rzpd.de/. All ESTs are deposited in dbEST (accession nos. BI385298–BI388632, BI378370–BI381823, BI381824–BI385297, and BI376198–BI378369). The consensus of the alignments of the C, D, Y, orthologs included in the CD/CDY groups that we describe are available for similarity searches at http://www.molgen.mpg.de/~amphioxus.]

Details

show
hide
Language(s): eng - English
 Dates: 2003-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 175372
ISI: 000183346500003
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genome Research
  Alternative Title : Genome Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (6) Sequence Number: - Start / End Page: 1056 - 1066 Identifier: ISSN: 1088-9051