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  Expression of galectin-3 in skeletal tissues is controlled by Runx2

Stock, M., Schäfer, H., Stricker, S., Gross, G., Mundlos, S., & Otto, F. (2003). Expression of galectin-3 in skeletal tissues is controlled by Runx2. Journal of Biological Chemistry, 278(19), 17360-17367. doi:10.1074/jbc.M207631200.

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Genre: Journal Article
Alternative Title : J Biol Chem

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 Creators:
Stock, Michael, Author
Schäfer, Henning, Author
Stricker, Sigmar1, Author           
Gross, Gerhard, Author
Mundlos, Stefan1, Author           
Otto, Florian, Author
Affiliations:
1Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

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 Abstract: The -galatoside-specific lectin galectin-3 is expressed in vivo in osteoblasts as well as in epiphyseal cartilage. Here we show that in vitro, galectin-3 expression is up-regulated in the preosteoblastic cell line MC3T3-E1 during the matrix maturation stage of the osteoblast developmental sequence. Expression persists into late differentiation stages when the mature osteoblastic phenotype is established. The skeletal expression pattern of galectin-3 overlaps at many sites with that of the transcription factor Runx2. Runx2 is a key regulator of osteoblast development and necessary for chondrocyte differentiation in the growth plate. Both human and mouse galectin-3 promoters contain putative Runx-binding sites. The constitutive or inducible forced expression of Runx2 is sufficient for the onset of galectin-3 transcription in the mesenchymal precursor cell line C3H10T1/2. Moreover, Runx2 is able to bind to at least two sites in the galectin-3 promoter region. The crucial role of Runx2 was confirmed in Runx2-deficient mice, which are devoid of galectin-3 expression in skeletal cells. The overlapping expression pattern of galectin-3 with the other two members of the Runt family of transcription factors (Runx1 and Runx3) points to a potential regulation of the galectin-3 gene (LGALS3) by these factors in hematopoietic, skin, and dorsal root ganglial cells.

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Language(s): eng - English
 Dates: 2003-05
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 178593
DOI: 10.1074/jbc.M207631200
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Title: Journal of Biological Chemistry
  Alternative Title : J Biol Chem
Source Genre: Journal
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Pages: - Volume / Issue: 278 (19) Sequence Number: - Start / End Page: 17360 - 17367 Identifier: ISSN: 0021-9258
ISSN: 1083-351X