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  Structure of a viral DNA gatekeeper at 10 angstrom resolution by cryo-electron microscopy

Orlova, E. V., Gowen, B., Dröge, A., Stiege, A., Weise, F., Lurz, R., et al. (2003). Structure of a viral DNA gatekeeper at 10 angstrom resolution by cryo-electron microscopy. EMBO Journal, 22(6), 1255-1262.

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Genre: Journal Article
Alternative Title : Embo J.

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 Creators:
Orlova, Elena V., Author
Gowen, Brent, Author
Dröge, Anja1, Author           
Stiege, Asita2, Author           
Weise, Frank3, Author
Lurz, Rudi3, Author
van Heel, Marin, Author
Tavares, Paulo3, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              
3Max Planck Society, ou_persistent13              

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Free keywords: bacteriophage SPP1; connector; cyclical oligomers; electron cryo-microscopy; portal protein
 Abstract: In tailed bacteriophages and herpes viruses, the viral DNA is packaged through the portal protein channel. Channel closure is essential to prevent DNA release after packaging. Here we present the connector structure from bacteriophage SPP1 using cryo-electron microscopy and single particle analysis. The multiprotein complex comprises the portal protein gp6 and the head completion proteins gp15 and gp16. Although we show that gp6 in the connector has a fold similar to that of the isolated portal protein, we observe conformational changes in the region of gp6 exposed to the DNA-packaging ATPase and to gp15. This reorganization does not cause closure of the channel. The connector channel traverses the full height of gp6 and gp15, but it is closed by gp16 at the bottom of the complex. Gp16 acts as a valve whose closure prevents DNA leakage, while its opening is required for DNA release upon interaction of the virus with its host.

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Language(s): eng - English
 Dates: 2003-03-17
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 173724
ISI: 000181546000003
 Degree: -

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Title: EMBO Journal
  Alternative Title : Embo J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 22 (6) Sequence Number: - Start / End Page: 1255 - 1262 Identifier: ISSN: 0261-4189