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  Sample preparation protocols for MALDI-MS of peptides and oligonucleotides using prestructured sample supports

Nordhoff, E., Schurenberg, M., Thiele, G., Luebbert, C., Kloeppel, K.-D., Theiss, D., et al. (2003). Sample preparation protocols for MALDI-MS of peptides and oligonucleotides using prestructured sample supports. International Journal of Mass Spectrometry, 226(1), 163-180. doi:10.1016/S1387-3806(02)00978-8.

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Genre: Journal Article
Alternative Title : Int. J. Mass Spectrom.

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 Creators:
Nordhoff, Eckhard1, Author           
Schurenberg, Martin, Author
Thiele, Gabriela, Author
Luebbert, Christine, Author
Kloeppel, Klaus-Dieter2, Author           
Theiss, Dorothea, Author
Lehrach, Hans1, Author           
Gobom, Johan1, Author           
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: MALDI, sample preparation, proteomics, oligonucleotide, peptide
 Abstract: This contribution provides a set of protocols for MALDI-MS sample preparation of peptides and oligonucleotides on prestructured sample supports. The protocols have been optimized for high detection sensitivity, robust performance, ease of use, and include sample purification and concentration. Some protocols were optimized for manual preparation of individual samples. Others were developed for the use of automated pipetting stations and optimized for high throughput protein identification and DNA sequence analysis.

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Language(s): eng - English
 Dates: 2003-03-15
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 175165
ISI: 000181270000011
DOI: 10.1016/S1387-3806(02)00978-8
 Degree: -

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Title: International Journal of Mass Spectrometry
  Alternative Title : Int. J. Mass Spectrom.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 226 (1) Sequence Number: - Start / End Page: 163 - 180 Identifier: ISSN: 1387-3806