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  Facilities and methods for the high-throughput crystal structural analysis of human proteins

Heinemann, U., Buessow, K., Mueller, U., & Umbach, P. (2003). Facilities and methods for the high-throughput crystal structural analysis of human proteins. Accounts of Chemical Research, 36(3), 157-163. doi:10.1021/ar010129t S0001-4842(01)00129-7.

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Genre: Journal Article
Alternative Title : Accounts Chem. Res.

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 Creators:
Heinemann, Udo, Author
Buessow, Konrad1, Author              
Mueller, Uwe, Author
Umbach, Patrick, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: Facilities and methods for the high-throughput crystal structure analysis of human proteins are described as recently established in the Protein Structure Factory, a Berlin-area structural genomics project. Genes encoding human proteins are expressed in either recombinant Escherichia coli or yeast (Saccharomyces cerevisiae or Pichia pastoris). To facilitate and standardize protein purification, the target proteins are produced with various tags for affinity chromatography. For high-throughput crystallization, a robotic station is being set up that has the capacity to handle 960 000 experiments simultaneously. The resulting protein crystals will be subjected to X-ray diffraction experiments at the third-generation synchrotron storage ring BESSY where protein crystallography beamlines are currently under construction. The Protein Structure Factory's strategy for high-throughput production and structure analysis of human proteins is evaluated based on first results.

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Language(s): eng - English
 Dates: 2003-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 173932
ISI: 000181706900002
DOI: 10.1021/ar010129t S0001-4842(01)00129-7
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Title: Accounts of Chemical Research
  Alternative Title : Accounts Chem. Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 36 (3) Sequence Number: - Start / End Page: 157 - 163 Identifier: ISSN: 0001-4842