English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Downregulation of putative tumor suppressor gene TSC-22 in human brain tumors

Shostak, K. O., Dmitrenko, V. V., Garifulin, O. M., Rozumenko, V. D., Khomenko, O. V., Zozulya, Y. A., et al. (2003). Downregulation of putative tumor suppressor gene TSC-22 in human brain tumors. Journal of Surgical Oncology, 82(1), 57-64.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : J. Surg. Oncol.

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Shostak, Katharina O., Author
Dmitrenko, Vladimir V., Author
Garifulin, Oleg M., Author
Rozumenko, Vladimir D., Author
Khomenko, Olexiy V., Author
Zozulya, Yuiry A., Author
Zehetner, Guenther1, Author              
Kavsan, Vadym M., Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Content

show
hide
Free keywords: glioblastoma multiforme, astrocytoma, differential hybridization, high-density cDNA filter arrays, differential expression
 Abstract: Background and Objectives: Our objective was to identify differentially expressed genes involved in the pathogenesis of glioblastoma multiforme (GBM). Methods: Screening of arrayed human fetal brain and human postnatal brain cDNA libraries was performed by differential hybridization with glioblastoma multiforme and human normal brain cDNAs. Results: Repeated differential hybridization of more than 100 cDNA clones selected by primary screening and analysis of RNA from adult normal brain and glial tumors showed 16 nucleotide sequences differentially expressed between normal brain and brain tumors. Among others, decreased content in astrocytic tumors was determined for TSC-22 mRNA corresponding to cDNA in the ICRFp507J1041 clone from human fetal brain cDNA library. Northern blot hybridization of RNA from different human brain tumors showed very low amounts of TSC-22 mRNA in most investigated samples of GBM, anaplastic astrocytoma, and some other tumors. Complete lack of expression of TSC-22 occurred in one sample of anaplastic astrocytoma, as well as in meningioma, brain sarcoma, sarcomatous meningioma, and oligodendroglioma. The differential expression of TSC-22 gene was confirmed by semiquantitative RT-PCR in 15 samples of astrocytomas WHO grade II-IV and three samples of normal brain. Conclusions: Significantly decreased levels of TSC-22 mRNA in human brain and salivary gland tumors and antiproliferative role of TSC-22 strongly suggest a tumor suppressor role for TSC-22.

Details

show
hide
Language(s): eng - English
 Dates: 2003-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 194743
ISI: 000180240500008
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Surgical Oncology
  Alternative Title : J. Surg. Oncol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 82 (1) Sequence Number: - Start / End Page: 57 - 64 Identifier: ISSN: 0022-4790