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  Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC

Weigel, C., & Seitz, H. (2002). Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC. Molecular Microbiology, 46(4), 1149-1156.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8B85-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8B86-9
Genre: Journal Article
Alternative Title : Mol Micrbiol

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 Creators:
Weigel, Christoph1, Author
Seitz, Harald2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: We analysed the enzymatic activity (strand dis-placement) of the Escherichia coli DnaB helicase on a mirror-image pair of oligonucleotide-based substrates mimicking the unwound replication origin oriC. Loading of the helicase complex occurred exclusively to the single-stranded 'lower strand' part of the substrates. Full helicase activity required DnaA bound to the double-stranded part of the substrates (oriC DnaA box R1) and to their single-stranded 'upper strand' part. We assume that in vivo DnaA also loads the first of two helicase complexes - required for the assembly of two replication forks - to the lower strand of oriC during initiation of bidirectional chromosome replication in E. coli.

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Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 27212
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Title: Molecular Microbiology
  Alternative Title : Mol Micrbiol
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 46 (4) Sequence Number: - Start / End Page: 1149 - 1156 Identifier: -