English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Analysis of differential gene expression in stretched podocytes: osteopontin enhances adaptation of podocytes to mechanical stress

Endlich, N., Sunohara, M., Nietfeld, W., Wolski, E. W., Schiwek, D., Kränzlin, B., et al. (2002). Analysis of differential gene expression in stretched podocytes: osteopontin enhances adaptation of podocytes to mechanical stress. The FASEB Journal, 16(13), 1850-1852.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : FASEB J

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Endlich, Nicole, Author
Sunohara, Masataka, Author
Nietfeld, Wilfried1, Author              
Wolski, Eryk W.2, Author
Schiwek, Daniel, Author
Kränzlin, Bettina, Author
Gretz, Norbert, Author
Kriz, Wilhelm, Author
Eickhoff, Holger, Author
Endlich, Karlhans, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: Glomerular hypertension · nephron degeneration · cDNA array · actin cytoskeleton · DOCA-salt
 Abstract: Glomerular hypertension is a major determinant advancing progression to end-stage renal failure. Podocytes, which are thought to counteract pressure-mediated capillary expansion, are increasingly challenged in glomerular hypertension. Studies in animal models of glomerular hypertension indicate that glomerulosclerosis develops from adhesions of the glomerular tuft to Bowman's capsule due to progressive podocyte loss. However, the molecular alterations of podocytes in glomerular hypertension are unknown. In this study, we determined the changes in gene expression in podocytes induced by mechanical stress in vitro (cyclic biaxial stretch, 0.5 Hz, 5% linear strain, 3 days) using cDNA arrays (6144 clones). Sixteen differentially regulated genes were identified, suggesting alterations of cell-matrix interaction, mitochondrial/metabolic function, and protein synthesis/degradation in stretched podocytes. The transcript for the matricellular protein osteopontin (OPN) was most strongly up-regulated by stretch (approximately threefold). By reverse transcriptase-polymer chain reaction, up-regulation of OPN mRNA was also detected in glomeruli of rats treated for 2.5 wk with desoxycorticosterone acetate-salt, an animal model of glomerular hypertension. In cultured podocytes, OPN coating induced a motile phenotype increasing actin nucleation proteins at cell margins and reducing stress fibers and focal adhesions. Intriguingly, additional OPN coating of collagen IV-coated membranes accelerated stretch-induced actin reorganization and markedly diminished podocyte loss at higher strain. This study delineates the molecular response of podocytes to mechanical stress and identifies OPN as a stretch-adapting molecule in podocytes.

Details

show
hide
Language(s): eng - English
 Dates: 2002-09-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 27791
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The FASEB Journal
  Alternative Title : FASEB J
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 16 (13) Sequence Number: - Start / End Page: 1850 - 1852 Identifier: ISSN: 0892-6638