English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A Novel Yeast Silencer: The 2µ Origin of Saccharomyces cerevisiae Has HST3-, MIG1- and SIR-Dependent Silencing Activity

Grünweller, A., & Ehrenhofer-Murray, A. E. (2002). A Novel Yeast Silencer: The 2µ Origin of Saccharomyces cerevisiae Has HST3-, MIG1- and SIR-Dependent Silencing Activity. Genetics, 162(1), 59-71.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8BB2-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8BB3-5
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Grünweller, Arnold1, Author
Ehrenhofer-Murray, Ann E.2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433554              

Content

show
hide
Free keywords: -
 Abstract: Silencing in Saccharomyces cerevisiae is found at the mating-type loci HMR and HML, in subtelomeric regions, and at the rDNA locus. Repressed chromatin is built up by the recruitment of the Sir proteins via their interaction with DNA-binding proteins that bind to silencers. Here, we have performed a genetic screen for novel sequence elements within the yeast genome that display silencing activity. We isolated as a novel silencer element the origin of replication from the endogenous 2µ plasmid (2µARS). 2µARS-mediated silencing was dependent upon the Sir proteins, the origin recognition complex (ORC), and Hst3, a Sir2 histone deacetylase homolog, suggesting that it constituted a novel class of silencing in yeast. Moreover, 2µARS carried a binding site for Mig1, a transcriptional repressor of glucose-regulated genes. Both the Mig1-binding site and the MIG1 gene were necessary for full silencing activity of 2µARS. Furthermore, Hst3 was physically present at 2µARS in a silencing context as well as at the endogenous 2µ plasmid. Also, Hst3 regulated the repression of the flipase gene, although this was likely an indirect effect of HST3 on FLP1 expression.

Details

show
hide
Language(s): eng - English
 Dates: 2002-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 26950
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genetics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 162 (1) Sequence Number: - Start / End Page: 59 - 71 Identifier: -