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Free keywords:
Matrix assisted laser desorption; ionization-time of flight-mass spectrometry; Minimal protein identifier; Protein arrays; Proteomics; Two-dimensional gel electrophoresis
Abstract:
We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a minimal protein Identifier (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.