English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit

Blaha, G., Burkhardt, N., & Nierhaus, K. H. (2002). Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit. Biophysical Chemistry, 96(2-3), 153-161.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Blaha, Gregor1, Author           
Burkhardt, Nils2, Author
Nierhaus, Knud H.1, Author           
Affiliations:
1Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              
2Max Planck Society, ou_persistent13              

Content

show
hide
Free keywords: Protein synthesis; Ribosome; Activation energy; Association of ribosomal subunits
 Abstract: Association of ribosomal subunits is an essential reaction during the initiation phase of protein synthesis. Optimal conditions for 70S formation in vitro were determined to 20 mM Mg2+ and 30 mM K+. Under these conditions, the association reaction proceeds with first order kinetics, suggesting a conformational change to be the rate-limiting step. 70S formation separates into two sub-reactions, the adaptation of the ribosomal subunits to the association conditions and the association step itself. The activation energy of the process was determined to 78 kJ/mol and revealed to be required exclusively for the adaptation of the small subunit, rather than the large subunit or the association step. The presence of mRNA [poly(U)] together with cognate AcPhe-tRNA, accelerates the association rate significantly, forming a well-defined 70S peak in sucrose gradient profiles. mRNA alone provokes an equivalent acceleration, however, the resulting 70S couple impresses as an ill-defined, broad peak, probably indicating the readiness of the ribosome for tRNA binding, upon which the ribosome flips into a defined state.

Details

show
hide
Language(s): eng - English
 Dates: 2002-02-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 24717
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 96 (2-3) Sequence Number: - Start / End Page: 153 - 161 Identifier: -