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  Protein Array Technology: The Tool to Bridge Genomics and Proteomics

Eickhoff, H., Konthur, Z., Lueking, A., Lehrach, H., Walter, G., Nordhoff, E., et al. (2002). Protein Array Technology: The Tool to Bridge Genomics and Proteomics. Advances in Biochemical Engineering / Biotechnology, 77, 103-112.

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 Creators:
Eickhoff, Holger, Author
Konthur, Zoltán1, Author           
Lueking, Angelika2, Author
Lehrach, Hans3, Author           
Walter, Gerald2, Author
Nordhoff, Eckhard3, Author           
Nyarsik, Lajos2, Author
Büssow, Konrad3, Author           
Affiliations:
1In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479653              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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Free keywords: protein array, expression profiling, automation, serum screening, antibody profiling, protein purification
 Abstract: The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are much more difficult to produce and to handle. Outside of a narrow range of environmental conditions, proteins will denature, lose their three-dimensional structure and a lot of their specificity and activity. The chapter describes the pitfalls and challenges in Protein Microarray technology to produce native and functional proteins and store them in a native and special environment for every single spot on an array, making applications like antibody profiling and serum screening possible not only on denatured arrays but also on native protein arrays.

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Language(s): eng - English
 Dates: 2002
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 27147
 Degree: -

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Title: Advances in Biochemical Engineering / Biotechnology
Source Genre: Journal
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Pages: - Volume / Issue: 77 Sequence Number: - Start / End Page: 103 - 112 Identifier: -