English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Quantitative intensity-based FRET approaches-A comparative snapshot.

Zeug, A., Woehler, A., Neher, E., & Ponimaskin, E. G. (2012). Quantitative intensity-based FRET approaches-A comparative snapshot. Biophysical Journal, 103(9), 1821-1827. doi:10.1016/j.bpj.2012.09.031.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8D1D-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-47AB-7
Genre: Journal Article

Files

show Files
hide Files
:
1586520.pdf (Publisher version), 184KB
Name:
1586520.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Zeug, A., Author
Woehler, A.1, Author              
Neher, E.1, Author              
Ponimaskin, E. G., Author
Affiliations:
1Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

Content

show
hide
Free keywords: -
 Abstract: Förster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically encoded FRET-based biosensors. A variety of procedures have been described for measuring FRET efficiency or the relative abundance of donor-acceptor complexes, based on analysis of the donor fluorescence lifetime or the spectrally resolved fluorescence intensity. The latter methods are preferable if one wants to not only quantify the apparent FRET efficiencies but also calculate donor-acceptor stoichiometry and observe fast dynamic changes in the interactions among donor and acceptor molecules in live cells. This review focuses on a comparison of the available intensity-based approaches used to measure FRET. We discuss their strengths and weaknesses in terms of FRET quantification, and provide several examples of biological applications.

Details

show
hide
Language(s): eng - English
 Dates: 2012-11-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bpj.2012.09.031
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biophysical Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 103 (9) Sequence Number: - Start / End Page: 1821 - 1827 Identifier: -