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  NMR in the SPINE structural proteomics project.

Ab, E., Atkinson, A. R., Banci, L., Bertini, I., Ciofi-Baffoni, S., Brunner, K., et al. (2006). NMR in the SPINE structural proteomics project. Acta Crystallographica Section D-Biological Crystallography, 62(10), 1150-1161. doi:10.1107/S0907444906032070.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-7096-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C7DB-4
Genre: Journal Article

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 Creators:
Ab, E., Author
Atkinson, A. R., Author
Banci, L., Author
Bertini, I., Author
Ciofi-Baffoni, S., Author
Brunner, K., Author
Diercks, T., Author
Dötsch, V., Author
Engelke, F., Author
Folkers, G. E., Author
Griesinger, C.1, Author              
Gronwald, W., Author
Günther, U., Author
Habeck, M., Author
De Jong, R. N., Author
Kalbitzer, H. R., Author
Kieffer, B., Author
Leeflang, B. R., Author
Loss, S., Author
Luchinat, C., Author
Marquardsen, T., AuthorMoskau, D., AuthorNeidig, K. P., AuthorNilges, M., AuthorPiccioli, M., AuthorPierattelli, R., AuthorRieping, W., AuthorSchippmann, T., AuthorSchwalbe, H., AuthorTravé, G., AuthorTrenner, J., AuthorWöhnert, J., AuthorZweckstetter, M.2, Author              Kaptein, R., Author more..
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              
2Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              

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 Abstract: This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe (SPINE) consortium. Focusing on the development of high-throughput (HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless13C-direct detection methods and inferential structure determinations (ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.

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Language(s): eng - English
 Dates: 2006-10-042006-10
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S0907444906032070
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Title: Acta Crystallographica Section D-Biological Crystallography
Source Genre: Journal
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Pages: - Volume / Issue: 62 (10) Sequence Number: - Start / End Page: 1150 - 1161 Identifier: -