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  Indole-3-acetaldoxime-derived compounds restrict root colonization in the beneficial interaction between Arabidopsis roots and the endophyte Piriformospora indica

Nongbri, P. L., Michal Johnson, J., Sherameti, I., Glawischnig, E., Halkier, B. A., & Oelmüller, R. (2012). Indole-3-acetaldoxime-derived compounds restrict root colonization in the beneficial interaction between Arabidopsis roots and the endophyte Piriformospora indica. Molecular Plant-Microbe Interactions, 25(9), 1186-1197. doi:10.1094/MPMI-03-12-0071-R.

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Nongbri, Pyniarlang L., Author
Michal Johnson, Joy1, Author           
Sherameti, Irena, Author
Glawischnig, Erich, Author
Halkier, Barbara Ann, Author
Oelmüller, Ralf, Author
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1IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: The growth-promoting and root-colonizing endophyte Piriformospora indica induces camalexin and the expression of CYP79B2, CYP79B3, CYP71A13, PAD3, and WRKY33 required for the synthesis of indole-3-acetaldoxime (IAOx)-derived compounds in the roots of Arabidopsis seedlings. Upregulation of the mRNA levels by P. indica requires cytoplasmic calcium elevation and mitogen-activated protein kinase 3 but not root-hair-deficient 2, radical oxygen production, or the 3-phosphoinositide-dependent kinase 1/oxidative signal-inducible 1 pathway. Because P. indica–mediated growth promotion is impaired in cyp79B2 cyp79B3 seedlings, while pad3 seedlings—which do not accumulate camalexin—still respond to the fungus, IAOx-derived compounds other than camalexin (e.g., indole glucosinolates) are required during early phases of the beneficial interaction. The roots of cyp79B2 cyp79B3 seedlings are more colonized than wild-type roots, and upregulation of the defense genes pathogenesis-related (PR)-1, PR-3, PDF1.2, phenylalanine ammonia lyase, and germin indicates that the mutant responds to the lack of IAOx-derived compounds by activating other defense processes. After 6 weeks on soil, defense genes are no longer upregulated in wild-type, cyp79B2 cyp79B3, and pad3 roots. This results in uncontrolled fungal growth in the mutant roots and reduced performance of the mutants. We propose that a long-term harmony between the two symbionts requires restriction of root colonization by IAOx-derived compounds.

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 Dates: 2012-05-172012-09
 Publication Status: Published online
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 Identifiers: Other: IMPRS058
DOI: 10.1094/MPMI-03-12-0071-R
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Title: Molecular Plant-Microbe Interactions
Source Genre: Journal
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Publ. Info: St. Paul, MN : APS Press
Pages: - Volume / Issue: 25 (9) Sequence Number: - Start / End Page: 1186 - 1197 Identifier: ISSN: 0894-0282
CoNE: https://pure.mpg.de/cone/journals/resource/954925558505