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  mMaple: A photoconvertible fluorescent protein for use in multiple imaging modalities.

McEvoy, A. L., Hoi, H., Bates, M., Platonova, E., Cranfill, P. J., Baird, M. W., et al. (2012). mMaple: A photoconvertible fluorescent protein for use in multiple imaging modalities. PLoS One, 7(12): e51314. doi:10.1371/journal.pone.0051314.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-72D2-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C826-3
Genre: Journal Article

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Creators

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 Creators:
McEvoy, A. L., Author
Hoi, H., Author
Bates, M.1, Author              
Platonova, E., Author
Cranfill, P. J., Author
Baird, M. W., Author
Davidson, H. E., Author
Liphardt, J., Author
Campbell, R. E., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFPcontaining construct.

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Language(s): eng - English
 Dates: 2012-12-11
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0051314
 Degree: -

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Title: PLoS One
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 7 (12) Sequence Number: e51314 Start / End Page: - Identifier: -