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  Mapping the potential energy landscape of intrinsically disordered proteins at amino acid resolution

Ozenne, V., Schneider, R., Yao, M., Huang, J.-R., Salmon, L., Zweckstetter, M., et al. (2012). Mapping the potential energy landscape of intrinsically disordered proteins at amino acid resolution. Journal of the American Chemical Society, 134(36), 15138-15148. doi:10.1021/ja306905s.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-72FB-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3B74-2
Genre: Journal Article

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http://pubs.acs.org/doi/pdf/10.1021/ja306905s (Publisher version)
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 Creators:
Ozenne, V., Author
Schneider, R., Author
Yao, M., Author
Huang, J.-R, Author
Salmon, L., Author
Zweckstetter, M.1, Author              
Jensen, M. R., Author
Blackledge, M., Author
Affiliations:
1Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              

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 Abstract: Intrinsically disordered regions are predicted to exist in a significant fraction of proteins encoded in eukaryotic genomes. The high levels of conformational plasticity of this class of proteins endows them with unique capacities to act in functional modes not achievable by folded proteins, but also places their molecular characterization beyond the reach of classical structural biology. New techniques are therefore required to understand the relationship between primary sequence and biological function in this class of proteins. Although dependences of some NMR parameters such as chemical shifts (CSs) or residual dipolar couplings (RDCs) on structural propensity are known, so that sampling regimes are often inferred from experimental observation, there is currently no framework that allows for a statistical mapping of the available Ramachandran space of each amino acid in terms of conformational propensity. In this study we develop such an approach, combining highly efficient conformational sampling with ensemble selection to map the backbone conformational sampling of IDPs on a residue specific level. By systematically analyzing the ability of NMR data to map the conformational landscape of disordered proteins, we identify combinations of RDCs and CSs that can be used to raise conformational degeneracies inherent to different data types, and apply these approaches to characterize the conformational behavior of two intrinsically disordered proteins, the K18 domain from Tau protein and NTAIL from measles virus nucleoprotein. In both cases, we identify the enhanced populations of turn and helical regions in key regions of the proteins, as well as contiguous strands that show clear and enhanced polyproline II sampling.

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Language(s): eng - English
 Dates: 2012-08-202012
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/ja306905s
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 134 (36) Sequence Number: - Start / End Page: 15138 - 15148 Identifier: -