English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  STED nanoscopy with time-gated detection: Theoretical and experimental aspects.

Vicidomini, G., Schönle, A., Ta, H., Han, K. Y., Moneron, G., Eggeling, C., et al. (2013). STED nanoscopy with time-gated detection: Theoretical and experimental aspects. PLoS One, 8(1): e54421. doi:10.1371/journal.pone.0054421.

Item is

Files

show Files
hide Files
:
1602392.pdf (Publisher version), 2MB
Name:
1602392.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Vicidomini, G.1, Author           
Schönle, A.1, Author           
Ta, H.1, Author           
Han, K. Y.1, Author           
Moneron, G.1, Author           
Eggeling, C.1, Author           
Hell, S. W.1, Author                 
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: -
 Abstract: In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy using time-gated detection, pulsed excitation and continuous wave (CW) STED beams. We present a theoretical framework and experimental data that characterize the time evolution of the effective point-spread-function of a STED microscope and illustrate the physical basis, the benefits, and the limitations of time-gated detection both for CW and pulsed STED lasers. While gating hardly improves the effective resolution in the all-pulsed modality, in the CW-STED modality gating strongly suppresses low spatial frequencies in the image. Gated CW-STED nanoscopy is in essence limited (only) by the reduction of the signal that is associated with gating. Time-gated detection also reduces/suppresses the influence of local variations of the fluorescence lifetime on STED microscopy resolution.

Details

show
hide
Language(s): eng - English
 Dates: 2013-01-18
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pone.0054421
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 8 (1) Sequence Number: e54421 Start / End Page: - Identifier: ISSN: 1932-6203