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  A20 and CYLD Do Not Share Significant Overlapping Functions during B Cell Development and Activation

Chu, Y., Soberon, V., Glockner, L., Beyaert, R., Massoumi, R., van Loo, G., et al. (2012). A20 and CYLD Do Not Share Significant Overlapping Functions during B Cell Development and Activation. JOURNAL OF IMMUNOLOGY, 189(9), 4437-4443. doi:10.4049/jimmunol.1200396.

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 Creators:
Chu, Yuanyuan1, Author              
Soberon, Valeria1, Author              
Glockner, Laura2, Author
Beyaert, Rudi2, Author
Massoumi, Ramin2, Author              
van Loo, Geert2, Author
Krappmann, Daniel2, Author
Schmidt-Supprian, Marc1, Author              
Affiliations:
1Schmidt-Supprian, Marc / Molecular Immunology and Signaltransduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565167              
2external, ou_persistent22              

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Free keywords: NF-KAPPA-B; SYSTEMIC-LUPUS-ERYTHEMATOSUS; DEUBIQUITINATING ENZYME CYLD; TUMOR-SUPPRESSOR GENE; RHEUMATOID-ARTHRITIS; UBIQUITIN; TNFAIP3; PROLIFERATION; HOMEOSTASIS; DEFICIENCY
 Abstract: The ubiquitin-editing enzyme A20 (TNFAIP3) and the deubiquitinase CYLD are central negative regulators of NF-kappa B signaling. Both can act by removing nonproteolytic K63-linked polyubiquitin chains from an overlapping set of signaling molecules. In B cells, A20 deficiency results in hyperactivity, loss of immune homeostasis, inflammation, and autoimmunity. The reported consequences of CYLD deficiency are controversial, ranging from an absence of effects to dramatic B cell hyperplasia. These differences could be due to varying compensation for the loss of CYLD function by A20. Therefore, to explore potential overlapping physiological functions between A20 and CYLD, we generated and characterized A20/CYLD double-deficient B cells. Interestingly, the lack of both A20 and CYLD did not exacerbate the developmental defects and hyperresponsive activity of A20-deficient B cells. In addition, the extent of B cell activation after in vitro stimulation with anti-CD40, LPS, and CpG was comparable in B cells lacking A20/CYLD and A20 alone. However, in response to BCR cross-linking, we observed small but reproducible additive effects of the lack of A20 and CYLD. Taken together, our results demonstrate that A20 and CYLD do not share significant functions during B cell development and activation. The Journal of Immunology, 2012, 189: 4437-4443.

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Language(s): eng - English
 Dates: 2012-11-01
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000310200600030
DOI: 10.4049/jimmunol.1200396
 Degree: -

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Title: JOURNAL OF IMMUNOLOGY
Source Genre: Journal
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Publ. Info: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA : AMER ASSOC IMMUNOLOGISTS
Pages: - Volume / Issue: 189 (9) Sequence Number: - Start / End Page: 4437 - 4443 Identifier: ISSN: 0022-1767