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  Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina

Fraccaroli, A., Franco, C. A., Rognoni, E., Filipa, N., Rehberg, M., Aszodi, A., et al. (2012). Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina. PLOS ONE, 7(10): e47488. doi:10.1371/journal.pone.0047488.

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 Creators:
Fraccaroli, Alessia1, Author
Franco, Claudio A.1, Author
Rognoni, Emanuel2, Author              
Filipa, Neto1, Author
Rehberg, Markus1, Author
Aszodi, Attila1, Author              
Wedlich-Söldner, Roland3, Author              
Pohl, Ulrich1, Author
Gerhardt, Holger1, Author
Montanez, Eloi1, Author              
Affiliations:
1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
3Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              

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Free keywords: F-ACTIN; IN-VIVO; ANGIOGENESIS; VASCULATURE; FILOPODIA; LIFEACT; CELLS; MICE
 Abstract: Angiogenesis requires coordinated changes in cell shape of endothelial cells (ECs), orchestrated by the actin cytoskeleton. The mechanisms that regulate this rearrangement in vivo are poorly understood - largely because of the difficulty to visualize filamentous actin (F-actin) structures with sufficient resolution. Here, we use transgenic mice expressing Lifeact-EGFP to visualize F-actin in ECs. We show that in the retina, Lifeact-EGFP expression is largely restricted to ECs allowing detailed visualization of F-actin in ECs in situ. Lifeact-EGFP labels actin associated with cell-cell junctions, apical and basal membranes and highlights actin-based structures such as filopodia and stress fiber-like cytoplasmic bundles. We also show that in the skin and the skeletal muscle, Lifeact-EGFP is highly expressed in vascular mural cells (vMCs), enabling vMC imaging. In summary, our results indicate that the Lifeact-EGFP transgenic mouse in combination with the postnatal retinal angiogenic model constitutes an excellent system for vascular cell biology research. Our approach is ideally suited to address structural and mechanistic details of angiogenic processes, such as endothelial tip cell migration and fusion, EC polarization or lumen formation.

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Language(s): eng - English
 Dates: 2012-10-24
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: PLOS ONE
Source Genre: Journal
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Publ. Info: 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA : PUBLIC LIBRARY SCIENCE
Pages: - Volume / Issue: 7 (10) Sequence Number: e47488 Start / End Page: - Identifier: ISSN: 1932-6203