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  A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface

Wakana, Y., van Galen, J., Meissner, F., Scarpa, M., Polishchuk, R. S., Mann, M., et al. (2012). A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface. EMBO JOURNAL, 31(20), 3976-3990. doi:10.1038/emboj.2012.235.

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Wakana, Yuichi1, Author
van Galen, Josse1, Author
Meissner, Felix2, Author              
Scarpa, Margherita1, Author
Polishchuk, Roman S.1, Author
Mann, Matthias2, Author              
Malhotra, Vivek1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: PROTEIN-KINASE-D; PLASMA-MEMBRANE PROTEINS; COATED VESICLES; MYOSIN-II; RAB GTPASES; ENDOPLASMIC-RETICULUM; MDCK CELLS; IN-VITRO; COMPLEX; SYNAPTOTAGMINSCARTS; Golgi membranes; myosin II; protein kinase D; transport carriers;
 Abstract: We have isolated a membrane fraction enriched in a class of transport carriers that form at the trans Golgi network (TGN) and are destined for the cell surface in HeLa cells. Protein kinase D (PKD) is required for the biogenesis of these carriers that contain myosin II, Rab6a, Rab8a, and synaptotagmin II, as well as a number of secretory and plasma membrane-specific cargoes. Our findings reveal a requirement for myosin II in the migration of these transport carriers but not in their biogenesis per se. Based on the cargo secreted by these carriers we have named them CARTS for CARriers of the TGN to the cell Surface. Surprisingly, CARTS are distinct from the carriers that transport vesicular stomatitis virus (VSV)-G protein and collagen I from the TGN to the cell surface. Altogether, the identification of CARTS provides a valuable means to understand TGN to cell surface traffic. The EMBO Journal (2012) 31, 3976-3990. doi:10.1038/emboj.2012.235; Published online 21 August 2012

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Language(s): eng - English
 Dates: 2012-10-17
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000310055400006
DOI: 10.1038/emboj.2012.235
 Degree: -

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Title: EMBO JOURNAL
Source Genre: Journal
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Publ. Info: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 31 (20) Sequence Number: - Start / End Page: 3976 - 3990 Identifier: ISSN: 0261-4189