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  Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast

Huang, J., Huang, Y., Yu, H., Subramanian, D., Padmanabhan, A., Thadani, R., et al. (2012). Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast. JOURNAL OF CELL BIOLOGY, 199(5), 831-847. doi:10.1083/jcb.201209044.

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 Creators:
Huang, Junqi1, Author
Huang, Yinyi1, Author
Yu, Haochen2, Author              
Subramanian, Dhivya1, Author
Padmanabhan, Anup1, Author
Thadani, Rahul1, Author
Tao, Yaqiong1, Author
Tang, Xie1, Author
Wedlich-Söldner, Roland2, Author              
Balasubramanian, Mohan K.1, Author
Affiliations:
1external, ou_persistent22              
2Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              

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Free keywords: CYTOKINETIC CONTRACTILE RING; POLARIZED CELL-GROWTH; UCS-DOMAIN PROTEIN; SCHIZOSACCHAROMYCES-POMBE; ANIMAL-CELLS; MYOSIN-II; GENE ENCODES; V MYOSINS; S-POMBE; DIVISION
 Abstract: In many eukaryotes, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring. Despite the central role of this ring in cytokinesis, the mechanism of F-actin assembly and accumulation in the ring is not fully understood. In this paper, we investigate the mechanism of F-actin assembly during cytokinesis in Schizosaccharomyces pombe using lifeact as a probe to monitor actin dynamics. Previous work has shown that F-actin in the actomyosin ring is assembled de novo at the division site. Surprisingly, we find that a significant fraction of F-actin in the ring was recruited from formin-Cdc12p nucleated long actin cables that were generated at multiple nonmedial locations and incorporated into the ring by a combination of myosin II and myosin V activities. Our results, together with findings in animal cells, suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables assembled elsewhere can contribute to ring assembly.

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Language(s): eng - English
 Dates: 2012-11-26
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000311492000012
DOI: 10.1083/jcb.201209044
 Degree: -

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Title: JOURNAL OF CELL BIOLOGY
Source Genre: Journal
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Publ. Info: 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA : ROCKEFELLER UNIV PRESS
Pages: - Volume / Issue: 199 (5) Sequence Number: - Start / End Page: 831 - 847 Identifier: ISSN: 0021-9525